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Event Details

  • Tuesday, February 26, 2019
  • 11:15 - 11:45

Engineering Supramolecular Plasmonic Structures for Detection of MERS- CoV Antigen and “Single Point” Amino Acids Mutation

Specific amino acid substitution has been associated with the instability of peptides and the formation of insoluble amyloid. Established experimental techniques to detect peptide mutations is either destructive or requires rigorous molecular labeling. We present a non-invasive and label-free detection method of amino acid substitution in primary peptide fragments. By optimizing a binary optical identification tag which encodes the Raman vibrational signals of amino acids and peptides, we can extract the structural components of the mutated peptide. A sensor design consisting of plasmonic nanostructures immobilized on an omniphobic surface is used to amplify the Raman signal. We demonstrate a tagging system which can detect a single point missense mutation in 25-35 Aβ fragment and a frameshift mutation in 1-42 Aβ fragment down to the picomole level. This work provides a potential strategy to produce optical identification tags which delivers sensitive and specific molecular information for biosensing.